Part:BBa_K5371203:Design

pESC-URA-MrBBS-BFP

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 4151
  Illegal EcoRI site found at 5819
  Illegal XbaI site found at 9760
  Illegal SpeI site found at 2229
  Illegal SpeI site found at 3459
  Illegal PstI site found at 395
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 4151
  Illegal EcoRI site found at 5819
  Illegal NheI site found at 6589
  Illegal SpeI site found at 2229
  Illegal SpeI site found at 3459
  Illegal PstI site found at 395
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 4151
  Illegal EcoRI site found at 5819
  Illegal BglII site found at 2194
  Illegal BglII site found at 3425
  Illegal BamHI site found at 6497
  Illegal XhoI site found at 6567
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 4151
  Illegal EcoRI site found at 5819
  Illegal XbaI site found at 9760
  Illegal SpeI site found at 2229
  Illegal SpeI site found at 3459
  Illegal PstI site found at 395
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 4151
  Illegal EcoRI site found at 5819
  Illegal XbaI site found at 9760
  Illegal SpeI site found at 2229
  Illegal SpeI site found at 3459
  Illegal PstI site found at 395
  Illegal NgoMIV site found at 1676
  Illegal AgeI site found at 6114
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 1085
  Illegal BsaI.rc site found at 7930
  Illegal SapI site found at 6847
  Illegal SapI.rc site found at 932
  

Design Notes

Stop codons of ERG20 and MrBBS were removed for continuous and complete expression of ERG20-linker-MrBBS to prevent a premature stop in this case. Length of the linker is adjusted to avoid frameshift, which may cause faulty translation. Codons of the linker is designed to avoid polarized amino acids or those with large residues.

Source

pESC-URA-MrBBS-BFP was fully synthesized by and purchased from Sangon Biotech (Shanghai) Co., Ltd.